Figure 2. miR-25 is necessary for adult NSPC prolifer-ation. NSPCs were transfected to knock down miR-106b, miR-93, or miR-25 or were transfected with a scrambled control oligonucleotide. Two days after transfection, NSPCs were incubated with EdU for 1 hour and then immediately fixed for analysis. (A) Representative photos for control knockdown and miR-25 knockdown. Scale bar: 100 μm. (B) Mean and SEM of the proportion of EdU+ cells for each condition, for experiments on 5 independent NSPC cultures (age 8-14 weeks, passage 3-7). Paired two-tailed t-test, **: p<0.01.