Figure 5. miR-106b~25 enhances neurogenesis in culture. NSPCs were infected with an empty control virus or virus to overexpress miR-106b~25. Three days after infection, NSPCs were placed in differentiation conditions for 7 days, and then stained for Tuj1, a marker of neurons. (A) Representative photos for each condition. Scale bar: 50 μm. (B) Mean and SEM of the proportion of Tuj1+ cells (total Tuj1+ cells/total DAPI-stained nuclei) normalized to control infection, for experiments on 4 independent NSPC cultures (age 12 weeks, passage 2), are shown. Paired two-tailed t-test, **: p<0.01.