Figure 3. Apoptotic figures in the lung tissues from 19 days old mutant embryos. (A) Apoptotic cells detection (TUNEL) assay on 19 days post-coitum embryonic lung sections showing a major increase in the number of apoptotic cells in Safb1-null/WrnΔhel/Δhel (or Safb1−/−/WrnΔhel/Δhel) embryos compared to the other genotypes. Healthy cells are stained in green (methyl green staining) and apoptotic cells are dark blue. Arrowheads point to representative apoptotic cells. Magnification 400X. (B) Average number of apoptotic figures per area of lung sections containing 1000 cells (n=3 embryos for each genotype; *: unpaired student t−test P-value < 0.05 compared to wild type Safb1+/+/Wrn+/+ animals).