Research Paper Volume 3, Issue 3 pp 277—290

Figure 5. Differential saturation density and growth properties of MEFs. (A) Growth curves of MEFs after 7-10 passages in culture (except for Safb1-null MEFs, which were measured at passage 24). Cells (5 × 10⁴) from wild type (Wrn^+/+/Safb1^+/+), Safb1-null (Safb1^−/−/Wrn^+/+), and Safb1-null/Wrn^Δhel/Δhel (Safb1^−/−/Wrn^Δhel/Δhel) embryos were plated in six-well plates as described in materials and methods. Cells were counted by trypan blue exclusion with a hemacytometer. (B) Histogram representing the growth rate of MEFs (from at least three embryos for each genotype) calculated from the growth curves in A. Bars represent the SEM. (Unpaired student t-test: *P < 0.000001 and **P < 0.026577 compared to wild type Wrn^+/+/Safb1^+/+animals). Growth rates were estimated as described in materials and methods.