Research Paper Volume 3, Issue 3 pp 277—290
The Werner syndrome helicase protein is required for cell proliferation, immortalization, and tumorigenesis in Scaffold Attachment Factor B1 deficient mice
Figure 9. DNA damage in MEFs. (A) Examples of nuclear foci detected by immunofluorescence with an antibody against γ-H2AX in MEFs of each genotype Magnification 600X. (B) Graph representing the extent of double stranded breaks detected with an antibody against γ-H2AX in MEFs of each genotype. The percentage of cells with more than 0, 10, and 20 γ-H2AX foci were computed from 100 MEFs established from three independent embryos for each genotype (total of 300 cells analyzed/genotype). Bars in the graph represent SEM.