Research Paper Volume 3, Issue 4 pp 395—406
Depletion of Ku70/80 reduces the levels of extrachromosomal telomeric circles and inhibits proliferation of ALT cells
Figure 6. Telomere FISH analysis of ALT cells expressing shRNAs for Ku70/80 or GFP. CCL75.1 cells expressing shRNAs for Ku70/80 or GFP were treated with 0.5 mg/ml of colcemid for 1.0 hour, harvested by trypsinization and swollen in 0.075M KCl for 20 min. The cells were fixed and washed with methanol:acetic acid (3:1) for three times and dropped into slides. Images shown are representative of more than 50 metaphases analyzed for each cell line.