Research Perspective Volume 3, Issue 3 pp 325—328

Does hypothalamic SIRT1 regulate aging?


Figure 1. Age-dependent decline in BAT content/ activity in perigonadal fat depot. The mRNA level of the brown adipose tissue (BAT)-specific gene uncoupling protein 1 (Ucp1) was used as a readout of BAT content/activity in perigonadal fat. Ucp1 mRNA levels in perigonadal fat of 2- and 7-month-old Sirt1loxP/loxP females fed on a standard chow (SC) diet and 3- and 7- month-old Sirt1loxP/loxP females fed on a high calorie (HC) diet [22] (n=9 in each group). Individual mRNA levels were normalized to 36B4 mRNA contents. HC-fed mice were fed on a SC diet up to 2 months of age and then switched and maintained on a HC diet. Note that perigonadal BAT content/activity similarly declines with age in SC and HC diet feeding conditions. Statistical analyses were done using two-tailed unpaired Student's t test. * P <0.05.