Research Perspective Volume 3, Issue 6 pp 635—642

SIRT1 and SIRT3 Deacetylate Homologous Substrates: AceCS1,2 and HMGCS1,2


Figure 3. SIRT1 Regulates the Acetylation Level of HMGCS1. (A) Recombinant HMGCS1 was expressed in HEK293 cells in the absence (control) or presence of nicotinamide (NAM, 50 mM), purified and measured for acetylation with an anti-acetyllysine antibody. (B) Expression vectors for wt SIRT1 or catalytically inactive SIRT1-H363Y were co-transfected into HEK293 cells with expression vectors for FLAG-tagged HMGCS1, and the levels of HMGCS1 acetylation were assessed.