Figure 5. FACS analysis of bidirectional TNT lysosomal transfer between EPC and control (A) or stressed (B) HUVEC (n=5). HUVEC were cultured on 60mm dishes and GC was added at each concentration for 72 h of co-culture with EPC. Native collagen I was added in control (0ug/ml) group instead of GC. Note that exposure to 50 ug/ml GC and above resulted in the increased exchange rate at 24 h of co-culture. Asterisks depict p< 0.05; n=4. NS, not significant.