Figure 4. Measurement of coupled mitochondrial ETC complex activities from young, middle aged and old WT and dwarf mouse pectoralis muscle mitochondria. CI-III and CII-III coupled enzyme activities were measured spectrophotometrically as described in Methods. All activity results are the average of 4 assays from the pooled samples ± SEM for each age group. Citrate synthase activities were used to normalize mitochondrial proteins. Activities for young (4-5 months), middle-aged (10-12 months), and old (20-26 months) WT and dwarf pectoralis CI-III and CII-III are plotted as follows: (A) CI-CIII coupled activity. Coefficients of variance for WT and dwarf were 8.2% and 6.1% (young), 7% and 12.9% (middle-age), and 4.7% and 5.4% (old), respectively. (B) CII-CIII coupled activity. Coefficients of variance for WT and dwarf were 2.9% and 6.3% (young), 6.2% and 5.9% (middle-age), and 10.2% and 2.9% (old), respectively. * - p<0.05 compared to young, ** - p<0.001 compared to young, and † - p<0.05 compared to middle-aged.