Research Paper Volume 3, Issue 8 pp 782—793

Mechanisms of chemotherapy-induced human ovarian aging: double strand DNA breaks and microvascular compromise


Figure 6. Comet assay with murine GV oocytes. Double strand DNA breaks were detected in murine GV oocytes using a single cell gel electrophoresis (Comet assay) after 24h culture with or without doxorubicin (10 μg/ml). Representative images of non-treated (A) and doxorubicin-treated (B) oocytes are shown. (C) Quantification of DNA damage in control versus doxorubicin treated oocytes; expressed as mean (± SD) % of DNA in the tail. P = 0.002 (t-test).