Research Paper Volume 3, Issue 10 pp 968—984

Characterization of spontaneously generated prion-like conformers in cultured cells


Figure 1. Detection of untreated and PK-treated PrP from three types of cultured cells with 3F4 and 1E4. A: Western blotting of cell lysates with or without PK-treatment at 25 μg/ml) probed with 3F4 (upper panel) and 1E4 (lower panel). WT: Lysates of cells expressing PrPWt. T183A: Lysates of cells expressing PrPT183A mutation. F198S: Lysates of cells expressing PrPF198S. T2: PrPSc type 2 control from sCJD. Di: Diglycosylated PrP. Mono181: PrP monoglycosylated at the first site. Mono197: PrP monoglycosylated at the second site. Un: Unglycosylated PrP. Comparison of affinities of 1E4 and 3F4 antibodies to the full-length and N-terminally truncated human PrP. The dashed-lines are used to align PrP bands on the blots.