Figure 2. Detection of oligomeric state of wild-type and mutant PrP. PrP from three types of cells were subjected to sucrose step gradient sedimentation. A: PrP in the fractions of the gradients was detected by Western blotting with 3F4. Upper panel: PrPWt. Middle panel: PrPT183A. Lower panel: PrPF198S. B: PrP in fractions 9-12 was treated with PK and PNGase F prior to Western blotting with 3F4 (upper panel) and 1E4 (lower panel).