Research Paper Volume 3, Issue 10 pp 968—984

Characterization of spontaneously generated prion-like conformers in cultured cells

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Figure 6. Digestion of wild-type and mutant PrP by Endo H. A: PrPWt, PrPT183A, or PrPF198S was treated with Endo H or PNGase F prior to Western blotting with 3F4. B: PrPF198S was treated with Endo H or PNGase F prior to Western blotting with Anti-C or anti-N. C: PrPSc from sCJD was treated with Endo H or PNGase F prior to Western blotting with anti-C or anti-N.