Research Paper Volume 3, Issue 9 pp 836—845

53BP1 contributes to a robust genomic stability in human fibroblasts


Figure 6. Correlation of micronuclei formation with maximal species lifespan. Skin fibroblasts prepared from adult animals of the indicated species under strict standardized culture conditions to ensure uniformity were treated with 1.5 μg/mL neocarzinostatin (NCS) for 2 hours to induce DNA damage. Damaged cells were allowed to recover for 72 hours, after which they were fixed and analyzed for micronucleus formation. Data indicating percentage of cells positive for micronuclei are pooled from two independent experiments. Spearman nonparametric analysis indicates an r value of −0.7982 with a P value of 0.006.