Research Paper Volume 3, Issue 9 pp 846—851

Figure 2. Plk1 expression is elevated in Aβ-treated neuronal PC12 cells. (A) PC12 cells were differentiated by treatment with NGF for 3d, incubated with Aβ_25-35 (10 μM) for 24 h in the presence or absence of BI 2536 (10 nM), and harvested for Western blotting with antibodies against Plk1 and β-actin, a loading control. (B) Samples prepared in the same way as in (A) were subjected to anti-Plk1 IP/kinase assay using GST-Orc2 as a substrate [28], followed by autoradiography. IP: immunoprecipitation.