Research Paper Volume 3, Issue 10 pp 1003—1017

Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle


Figure 2. GAPDH pool levels in plantaris/FHL lysate from old mice. Protein (30 μg) from total muscle cell lysates of plantaris/FHL from old mice (24-27 months) subjected to two hours of ischemia were separated by SDS-PAGE, transferred to PVDF membrane, and blotted with anti-GAPDH antibody. (A) Groups I, one day; II, 3 days, III, 5 days and IV, 7 days of reperfusion all showed statistically significant decreases in GAPDH immunoreactive pool levels. (B) A bar graph showing the statistical differences in GAPDH pool levels in response to 2 hours of ischemia and 1-7 days of reperfusion.