Research Paper Volume 3, Issue 10 pp 1003—1017

Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle


Figure 6. Western blot analysis of total tyrosine nitration of plantaris/FHL lysate from old mice following I/R. (A) Tyrosine nitration of whole cell lysate of glycolytic skeletal muscle from old mice (24-27 months) with 2 hours ischemia and variable lengths of reperfusion was examined by Western blot. C = contralateral control; Is = I/R; Group I = 1 day reperfusion; Group II = 3 days reperfusion; Group III = 5 days reperfusion, and Group IV = 7 days reperfusion. (B) Densitometric analysis shows statistically significant differences were observed at 1, 3, and 5 days of reperfusion.