Research Paper Volume 3, Issue 10 pp 1003—1017

Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle

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Figure 8. Tyrosine nitration of GAPDH in plantaris/FHL lysate from young mice. (A) Tyrosine nitration was assessed by Western blot of one dimensional gel electrophoresis. (B) Densitometric analysis shows nitration was significantly increased for GAPDH at 1 and 3 days of reperfusion, with the greatest increase at 3 days reperfusion. C = contralateral control; Is = I/R; Group I = no reperfusion; II = 1 day reperfusion; Group III = 3 days reperfusion; Group IV = 5 days reperfusion, and Group V = 7 days reperfusion. * = p < 0.05.