Figure 4. Age-predictions with the Epigenetic-Aging-Signature for the validation group. Age predictions were tested with eight independent datasets. Beta-values of the five CpG sites were retrieved and plotted against donor age (A). The beta-values were used for the linear regression models of the training-set to predict the age of the donors based on 5 CpG sites (R2=0.68) (B) or 3 CpG sites (R2=0.74) (C) of the Epigenetic-Aging-Signature. PB = peripheral blood; CB = cord blood; HPC = hematopoietic progenitor cells; MNC = mononuclear cells.