Figure 1. THC-induced nuclear accumulation of FOXO4. (A) Dose-dependent effects of THC on nuclear accumulation of FOXO4 in NIH-3T3-FOXO4 cells. Cy3 (red) and DAPI (blue) label FOXO4 and nuclei, respectively. (B) FOXO4 levels increase in nuclear fractions from NIH-3T3-FOXO4 cells. Lamin B was used as a nuclear marker and the Akt inhibitor LY294002 (LY) was used as a positive control. Anti-AFX (N-19) primary antibody was used in both analyses.