Figure 2. Time course of FOXO4 localization in response to THC. (A) FOXO4 protein levels increase in the nuclear fraction of NIH-3T3-FOXO4 cells within minutes of THC treatment. Lamin B was used as a nuclear marker, DMSO as a negative control. (B) Time course of FOXO4 nuclear translocation in NIH-3T3-FOXO4 cells following administration of THC. Red indicates FOXO4 protein. (C) The number of cells with nuclear FOXO was quantified at the indicated times following THC administration.