Research Paper Volume 3, Issue 11 pp 1098—1109

Tetrahydrocurcumin extends life span and inhibits the oxidative stress response by regulating the FOXO forkhead transcription factor


Figure 2. Time course of FOXO4 localization in response to THC. (A) FOXO4 protein levels increase in the nuclear fraction of NIH-3T3-FOXO4 cells within minutes of THC treatment. Lamin B was used as a nuclear marker, DMSO as a negative control. (B) Time course of FOXO4 nuclear translocation in NIH-3T3-FOXO4 cells following administration of THC. Red indicates FOXO4 protein. (C) The number of cells with nuclear FOXO was quantified at the indicated times following THC administration.