Research Paper Volume 4, Issue 1 pp 40—48
Age-related resistance of skeletal muscle-derived progenitor cells to SPARC may explain a shift from myogenesis to adipogenesis
Figure 3. Inhibition of SPARC expression by RNA silen-cing in young and old rat SMPCs. (A) The efficiency of silencing by siSPARC was evaluated by qPCR in SMPCs from young and old rats 2 days after siRNA transfection. Data are expressed as the mean ± SEM (n = 4, respectively). *p < 0.05 vs. control. (B) The efficiency of siSPARC silencing was evaluated by immunoblotting SMPC extracts prepared from young and old rats 2 days after siRNA transfection. (C) Quantitative analysis of immunoblotting data. Data are expressed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. control.