Research Paper Volume 4, Issue 1 pp 40—48

Figure 4. Effects of siSPARC on the adipogenic potential of SMPCs. (A) Representative Oil Red-O staining of SMPCs cultured for 4 days in differentiation medium (above), and quantitative analysis of Oil Red-O-positive area (below). Oil Red-O-positive areas were quantified as pixel values and the ratios to control values are graphed. Data are expressed as mean ± SEM (n = 4, respectively). *p < 0.05 vs. control. Scale bar = 200 μm. (B) Representative immunostaining of C/EBPα in SMPCs cultured for 4 days in differentiation media (above), and quantitative analysis of C/EBPα-positive cells (below). Nuclei were counterstained with Hoechst 33258. White arrowheads indicate C/EBPα-positive nuclei. Scale bar = 100 μm. The proportions of C/EBPα-positive cells were calculated and the ratios to control values are graphed. Data are expressed as mean ± SEM (n = 4, respectively). *p < 0.05 vs. control.