Research Paper Volume 4, Issue 1 pp 40—48
Age-related resistance of skeletal muscle-derived progenitor cells to SPARC may explain a shift from myogenesis to adipogenesis
Figure 6. Effects of exogenously added SPARC on differentiation of SMPCs with diminished levels of SPARC expression. SMPCs from young and old rats were cultured for 4 days in adipogenic media in the presence of BSA (20 μg/ml) or recombinant SPARC (20 μg/ml) 1 day after transfection with siSPARC. (A) Oil Red-O-positive areas were quantified as pixel values and the ratios to control values are graphed. Data are expressed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. BSA-treated cells. (B) Proportions of MHC-positive cells were calculated and ratios to control values are graphed. Data are expressed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. BSA treated cells.