Research Paper Volume 4, Issue 1 pp 40—48

Figure 8. Knockdown of ITGA5 expression in young SMPCs by siRNA transfection. (A) Efficiency of siRNA silencing was evaluated by qPCR in SMPCs from young rats 2 days after transfection with siItga5: Graphed data are expressed as mean ± SEM (n = 6, respectively). *p < 0.05 vs. control. (B) Quantitative analysis of relative fluorescence of ITGA5 in young SMPCs 2 days after transfection with siItga5. Graphed data are expressed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. control. Quantitative analysis of Oil Red-O staining results (C) and proportion of MHC-positive cells (D) of SMPCs at 5 days after siItga5 or siSPARC and siItga5 cotransfection. Graphed data are expressed as mean ± SEM (n = 3, respectively). Letters (a, b and c) indicate statistically significant differences (p < 0.05). Quantitative analysis of Oil Red-O staining results (E) and proportion of MHC-positive cells (F) of SMPCs cultured for 4 days in the presence of BSA (20 μg/ml) or recombinant SPARC (20 μg/ml) followed by cotransfection with siSPARC and siItga5. Graphed data are expres-sed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. control.