Research Paper Volume 4, Issue 11 pp 742—754

Aging affects epidermal Langerhans cell development and function and alters their miRNA gene expression profile


Figure 5. Aging regulates miRNA gene expression profiles in epidermal LCs. (A) LCs were isolated using AutoMACS with anti-MHCII-PE and anti-PE microbeadsfollowed by a cell sorter. The purity was confirmed to be ~98%.(B) Expression profiles of miRNAs in young and aged LCs from two individual experiments. (C) miRNA expression in LCs was significantly up- and downregulated in aged mice compared to that in young mice. (D) Changed miRNAs in aging were confirmed by single TaqMan RT-PCR.