Figure 2. Changes in ROS levels in WT andcat cells during exponential growth and chronological aging. DHR staining and FACS analysis was performed with exponentially growing cells (4 h after inoculation for Glc/AS, Glc/MA, Gly/AS and 16 h for Gly/MeOH/AS) (A) and chronologically aging cells grown on media containing Glc/AS (B), Gly/AS (C), Glc/MA (D) or Gly/MeOH/AS (E). Data represents mean ± SD, n = 3. * - P<0.05, ** - P<0.01 in student T test.