Research Paper Volume 5, Issue 3 pp 155—173

Figure 6. Allelic composition of PrP^Sc in fCJD^Ins+sPrPSc. (A) Western blot analysis of PrP precipitated by either 6H4 or g5p. PrP from S2 or P2 was precipitated with 6H4 or g5p, respectively. The precipitated PrP was subjected to deglycosylation with PNGase F and followed by SDS-PAGE and immunoblotting with anti-N antibody. Although there are different profiles of PrP precipitated by 6H4 in S2 and by g5p in P2, they exhibit two bands in both preparations containing an upper band corresponding to full-length PrP^Ins and a lower band corresponding to full-length PrP^Wt. (B) Quantitative analysis of allelic composition of PrP^Sc and PrP^C from either PrP^Ins or PrP^Wt. The intensities of PrP treated with PNGase F on the blot shown in A were analyzed by densitometry. The PrP species precipitated from S2 by 6H4 was considered as PrP^C while PrP precipitated from S2 and P2 by g5p was considered as PrP^Sc. Total PrP in fCJD^Ins+sPrPSc was composed of ~60% PrP^Wt and ~40% PrP^Ins. Approximately 62% PrP^Ins was converted into PrP^Sc while ~38% remained as PrP^C. In contrast, approximately 64% PrP^Wt remained as PrP^C while 36% was converted into PrP^Sc. PrP^Sc was composed of 52% PrP^Ins and 48% PrP^Wt while PrP^C was composed of 28% PrP^Ins and 72% PrP^Wt. These data represent averages from three independent experiments. *p < 0.05.