Figure 5. Adipocytes differentiated from HGPS iPSC derived MSCs show similar defects. (A) Schematic diagram of the experimental timeline starting from iPSCs, via MSCs, into ADs (adipocytes) with phase contrast pictures underneath each stage. Cell lines: Normal (168) and HGPS (167). (B) Images of Oil red O staining show red lipid droplets in AD168 and AD167 adipocytes at the third week of adipogenic differentiation. (C) Quantitative RT-PCR analysis of lamin A, progerin, early adipogenic genes C/EBPβ and C/EBPδ, and late adipogenic genes PPARγ2 and C/EBPα in normal AD168 (black) and HGPS AD167 (red) cells during three weeks adipogenesis from MSCs. n = 3. *p < 0.05, ** p < 0.01. (D) Fluorescence intensity of bodipy 493/503 staining at the third week of adipocyte differentiation. *p < 0.05.