Research Paper Volume 5, Issue 10 pp 770—781

PP2A inhibition results in hepatic insulin resistance despite Akt2 activation


Figure 6. Inhibition of hepatic PP2A results in inactivation of GS and activation of Phosphorylase. LB1-treatment resulted in increased phosphorylation (Ser641) and inactivation of GS (a) while increasing the ratio of active Phosphorylase a to inactive b (b) in the livers of fat-fed rats. Cantharidin-treatment in primary rat hepatocytes mimicked these effects and resulted in inactivation of GS (c) and activation of Phosphorylase (d). Data are averages of western blot quantifications ±SEM. * P<0.05. Representative western blots are shown.