Research Paper Volume 7, Issue 1 pp 14—25

Functional properties of bone marrow derived multipotent mesenchymal stromal cells are altered in heart failure patients, and could be corrected by adjustment of expansion strategies


Figure 6. Culturing strategy affects the phenotype of cellular population and the efficiency of in vitro expansion. (A) Kaplan-Meier survival curves for analysis of proliferation rate during BM-MMSC expansion in vitro under different culture conditions. The log-rank tests confirmed the significant difference for each pair of culturing conditions (p<0,001). (∉) n=43; (■) n=20; (▲) n=21. (B-D) The frequency of SMAα+ cells in cultures seeded at low density in both, normoxia and hypoxia is reduced compared to cultures seeded high density.