Figure 5. Levels of DNA strand breaks at base line and following IR exposure. (A) Fluorimetric quantitation of alkaline DNA unwinding carried out with lysates of untreated cells (open circles) and cells exposed to 3.8 Gy (closed circles). Values are normalised to total signal intensity corresponding to non-unwound DNA. (B) IR-dose-response curves determined as in (A). (C) Cross-comparison of base line H2AX phosphorylation (in the absence of VP16, same data as represented by open symbols in Fig.