Research Paper Volume 7, Issue 3 pp 205—216

The p53/miR-17/Smurf1 pathway mediates skeletal deformities in an age-related model via inhibiting the function of mesenchymal stem cells


Figure 2. BMMSCs from old mice express higher levels of senescence markers and lower osteoblast markers compared to young ones. Statistically analyzed values show the mean ± SD (n=10). * p < 0.05. (A) In vitro staining of the senescence-related marker ß-galactosidase in BMMSCs cultures derived from young and old mice. Quantitative analysis of the total number of positively stained cells. (B-C) Real-time PCR analyses on whole bone tissue extracts (B) and on BMMSCs (C) for the senescence-related genes p16, p21 and p53. Normalization to ß-actin. (D) The western blot showed that the protein level changed as the mRNA. (E) Alizarin red staining of BMMSCs from young and old mice osteogenically induced for 14 d. Cont = Control, OS = osteogenically induced. (F-G) Real-time PCR and western blot analyses on BMMSCs for the osteogenic markers Runx2, ALP, osterix. Normalization to ß-actin.