Research Paper Volume 7, Issue 3 pp 205—216

The p53/miR-17/Smurf1 pathway mediates skeletal deformities in an age-related model via inhibiting the function of mesenchymal stem cells


Figure 5. Up-regulation of miR-17 by miR-17 mimics reversed the effect of p53 on inhibiting osteogenic differentiation in old BMMSCs. miR-17 was stable upregulated in BMMSCs by miR-17 mimics (= miR-17 mimics). miRNA control (= miR Cont). Statistically analyzed values show the mean ± SD (n=10). * p < 0.05. (A) Osteogenic induction for 14 d of BMMSCs derived from old mice and subsequent alizarin red staining resulted in a heightened osteogenic differentiation of BMMSCs with previously upregulated miR-17 expression. (B-C) Real-time PCR and western blot analyses on old BMMSCs with miR-17 mimic treatment or miRNA control and with/without osteogenic induction for the osteogenic markers Runx2, ALP, osterix. Normalization to ß-actin. (D-E) Histological analysis (D) and corresponding statistical analysis (E) on osteoid formation of tissue sections from subcutaneous pockets on the backs of 6-week-old NOD/SCID mice with implanted HA/TCP ceramic particles mixed with BMMSCs from 16-month-old mice with/without miR-17 upregulation.