Figure 1. Overlapping effects of CR and ade4∆ on chronological lifespan and gene expression. (A) Semi-quantitative CLS assay showing the longevity effects for BY4741 (WT) under calorie restricted (CR) 0.5% glucose or non-restricted (NR) 2% glucose conditions, and with a non-restricted ade4∆ mutant. (B) Venn diagram showing numbers of differentially regulated genes that overlap between CR and ade4∆ conditions at day 8. (C) Scatter plot of gene expression fold-changes (log2) between CR and ade4∆ conditions. (D) Heat map of gene expression fold-changes (log2) induced by CR or ade4∆ relative to WT.