Figure 3. Overlapping effects of isonicotinamide (INAM) and expired CR media concentrate on CLS and gene expression. (A) Isonicotinamide (25 mM), extends CLS of WT yeast through a SIR2 independent mechanism. (B) Venn diagrams showing the number of genes found to be commonly up‐ or downregulated among multiple longlived conditions – CR conc., ade4∆, INAM, and CR– when compared to WT NR day 8 stationary cell populations. (C) Heat map of gene expression fold‐changes (log2) induced by INAM or the CR concentrate relative to WT. (D) Scatter plot of gene expression fold‐changes (log2) between day 8 cultures supplemented with the CR concentrate vs. INAM.