Figure 6. Testing efficacy of putative gene expression CLS extension biomarkers. (A) Single gene deletion mutants (mba1∆, bna2∆, and tor1∆) extend CLS and provide test cultures to evaluate the predictive value of the potential biomarkers identified through the microarray analysis. (B) Quantitative RT-PCR analysis of putative upregulated gene biomarkers in the mba1∆ and bna2∆ mutants. (C) Quantitative mRNA levels of ADD66, LEU3, and FYV6 are downregulated in all tested long-lived populations relative to WT at day 8.