Research Paper Volume 7, Issue 7 pp 500—512

Removal of growth hormone receptor (GHR) in muscle of male mice replicates some of the health benefits seen in global GHR−/− mice


Figure 1. Disruption of GHR gene in muscle. (A) DNA isolated from WAT (subcutaneous or SubQ, epididymal or Epi, retroperitoneal or Retro, and mesenteric or Mes depots), BAT, liver, skeletal muscle (quadriceps or Quad, gastrocnemius or Gast, and soleus or Sol), heart, kidney, spleen, and brain tissues was analyzed by PCR. The negative control (first lane on the left) is DNA isolated from liver of floxed control mice (WT), while the positive control (second lane on the left) is DNA isolated from the liver of global EIIaGHRKO mice. PCR primer locations are shown in part C of this figure. For each tissue, floxed controls (-C) are on the left while MuGHRKO (KO) are on the right. (B) Quantification of GHR mRNA levels in WAT, liver, skeletal muscle (quadriceps), and heart in floxed controls (n=6; white bars) and MuGHRKO mice (n=6; black bars) was performed by real time RT-PCR. (C) Schematic representation of Cre-Lox mediated disruption of the GHR gene. Primer locations (used for PCR in part A of this figure) are indicated with arrows. * indicates significant difference at p<0.05.