Figure 3. Expression of p38α and P-p38α in the gastrocnemius of aged (20 mos) wild type (WT) and DN-p38αAF/+ mice. (A-D) Immunohistofluorescence analysis of the levels of p38α and P-p38α in cross sections of the gastrocnemius of aged (20 mos) (A, B) WT and (C, D) DN-p38αAF/+ mice; The red immunofluorescence depicts pool levels of total p38α or total P-p38α using antibodies specific to p38α and P-p38α. The blue immunohistofluorescence depicts DAPI stained nuclei; Scale bar = 50 μm. (E) A bar graph presentation of the western blot data in (F). The data in (E) are depicted as relative OD vs. β-Actin values of Western blots; *p < 0.05, vs. corresponding WT. (F) Western blot (immunoblot) analysis of the total p38α levels and total P-p38α pool in (A, B) aged WT (20 mos) and (C, D) DN-p38αAF/+ mice.