Research Paper Volume 7, Issue 9 pp 648—660

Heart-specific Rpd3 downregulation enhances cardiac function and longevity


Figure 7. Extended lifespan in flies with heart-specific Rpd3 downregulation. (A) Heart-specific Gal4 expression levels during aging. The Gal4 expression was measured at the 2-day, 1-week, 4-week, and 7-week time points using the whole body's total RNA of flies with heart-specific Rpd3 downregulation (rpd3Ri/tinG4 and rpd3Ri/tinG4,UASG4). The Gal4 expression of the 2-day old rpd3Ri/tinG4 flies is set as log2 = 0 and other Gal4 expressions are compared from 3 - 5 independent RT-PCR experiments. (B) Oxidation stress assay of rpd3Ri/tinG4,UASG4 flies, which was normalized with the common control rpd3Ri/+ from five independent experiments. The data of rpd3Ri/tinG4 in Fig. 3A was indicated together for the comparison. (C) Differential expression of anti-aging genes in rpd3Ri/tinG4,UASG4 flies, which was obtained by comparison to the 2-day old rpd3Ri/tinG4 male flies (5 - 9 trials). The data was standardized against the common rpd3Ri/+ control (100%) in Fig. 4A. (D) Cardiac function throughout the lifespan of flies with heart-specific Rpd3 downregulation, of which heart rates were measured at 1, 3, 5 and 7-week time points. The percent changes in heart rate were represented from a comparison to the single transgene control (0%) at each time point (30 - 100 counting of heart rates). (E) The lifespan among adult male flies between the common control (rpd3Ri/+) and heart-specific Rpd3 downregulation (rpd3Ri/tinG4 or rpd3Ri/tinG4,UASG4).