Figure 7. Extended lifespan in flies with heart-specific Rpd3 downregulation. (A) Heart-specific Gal4 expression levels during aging. The Gal4 expression was measured at the 2-day, 1-week, 4-week, and 7-week time points using the whole body's total RNA of flies with heart-specific Rpd3 downregulation (rpd3Ri/tinG4 and rpd3Ri/tinG4,UASG4). The Gal4 expression of the 2-day old rpd3Ri/tinG4 flies is set as log2 = 0 and other Gal4 expressions are compared from 3 - 5 independent RT-PCR experiments. (B) Oxidation stress assay of rpd3Ri/tinG4,UASG4 flies, which was normalized with the common control rpd3Ri/+ from five independent experiments. The data of rpd3Ri/tinG4 in Fig.