Research Paper Volume 7, Issue 9 pp 664—672

Cellular senescence-like features of lung fibroblasts derived from idiopathic pulmonary fibrosis patients


Figure 2. Morphology analysis of lung fibroblasts by ImageStreamX. BF – bright field. Phalloidin, DAPI and DiD refer to staining of actin, nucleus and cell membrane, respectively. (A) Selected representative images. (B) Distribution of cell populations according to indicated criterion. The meaning of each variable is explained next to the images. Red lines indicate the cells in which actin is co-localized with cell membrane (score > 1.5). Note: IPF-PF vs. N-PF represent a more heterogeneous (more variable) cell population as evident by CV values (Table 1).