Figure 1. Immunoproteasome expression is increased in lung tissue of aged mice. (A) Overview over the proteasome active site nomenclature. (B) Representative H&E staining of lung from young and aged wildtype C57Bl/6 mice. Images from two individual animals are depicted per group. (C) RTqPCR analysis of proteasome subunit mRNA expression in young and aged wildtype mice. n = 8+SEM. (D) Western blot analysis of proteasome subunit expression in young and aged wildtype mice and quantification of protein expression relative to the β − actin loading control. Bar graphs show mean+SEM.