Figure 3. Effects of VMY on stress related proteins in MB cells. DAOY and D556 cells were treated with PVB or VMY. (A) Immunoblotting was performed for total and phosphorylated p38 and phosphorylated γ-H2AX following treatment for 6 or 18 hrs. (B) Immunofluorescence microscopy for γ-H2AX was performed on DAOY cells treated with 1 uM doxorubicin for 18 hrs and DAOY and D556 cells treated with 10 uM VMY for 18 hrs. DAPI was used to stain the nuclei. PVB; purvalanol B, Doxo; doxorubicin.