Research Paper Volume 7, Issue 10 pp 854—867

The p53 tumor suppressor protein protects against chemotherapeutic stress and apoptosis in human medulloblastoma cells

Figure 5. VMY alters the subcellular localization of p53. (A) Immunoblotting for MDM2 following exposure of D556 cells to PVB and VMY for the times indicated. β-actin was used as a loading control. (B) Immunofluorescence microscopy for p53 subcelluar localization was performed on DAOY (left panels) and D556 (right panels). DAPI was used to stain the nuclei. PVB; purvalanol B.