Figure 5. VMY alters the subcellular localization of p53. (A) Immunoblotting for MDM2 following exposure of D556 cells to PVB and VMY for the times indicated. β-actin was used as a loading control. (B) Immunofluorescence microscopy for p53 subcelluar localization was performed on DAOY (left panels) and D556 (right panels). DAPI was used to stain the nuclei. PVB; purvalanol B.