Figure 1. Circular RNA identification in skeletal muscle. (A) Workflow of the preparation and analysis of circRNAs. After homogenization of skeletal muscle samples, total RNA was extracted and digested with RNase R to degrade linear RNA. Following sequencing (RNA-Seq), sequences were processed, aligned to linear transcripts, assembled into collections of known and novel circRNAs, and annotated. (B) Representative UCSC genome browser views of mmu_circ_017332 (top), mmu_circ_014269 (middle), and the intronic circ-RNA mmu_circ_006990 (bottom), including fine-scale alternate splicing patterns that generate additional circRNA isoforms.