Figure 6. Metabolomic analysis of plasma and cortex demonstrate that alterations in biological pathways between young SAMP8 and old SAMP8 mice are partially rescued by J147. Plasma (A) and cortex (B) heatmaps of the biochemicals found significantly modified, organized by major biological groups. Scaled expression value (Z-score) is plotted in red-blue color scale with red indicating high expression and blue indicating low expression. Venn diagrams illustrating shared and uniquely affected metabolites in plasma (C) and cortex (D). Correlation of metabolite levels altered in Young/Old and Old+J147/Old in plasma (E) and cortex (F) (units are -log(fold change)). (G) Selection of relevant biochemicals changed between young and old SAMP8 mice affected by J147, including γ-glutamyl amino acids, dipeptides, BCAAs, acyl carnitines and PUFAs in the plasma, and metabolites related to neurotransmission and energetic pathways in the cortex. One-way ANOVA followed by Tukey-Kramer post-hoc test (n = 5/group). Values are expressed as box-and-whisker plots.