Research Paper Volume 7, Issue 11 pp 986—999

Screening of a kinase library reveals novel pro-senescence kinases and their common NF-κB-dependent transcriptional program


Figure 1. Profiles of SASP component induction by anti-proliferative kinases. (A-D) IMR-90 normal human fibroblasts were infected with a control vector or the indicated kinase-encoding retroviral vector. Five days after infection, RNAs were prepared and the indicated SASP-component transcript was quantified by RT-qPCR. Results were normalized with respect to the level of ACTB mRNA. (E) Relative (log2) fold change induction for each cytokine is represented on a heatmap versus the inducing kinase. (F) Correlograms and correlation matrix (non-parametric Spearman method) showing pairwise correlations between fold change induction datasets. Rho values and associated p-values are displayed.