Research Paper Volume 7, Issue 12 pp 1143—1155

NSun2 delays replicative senescence by repressing p27 (KIP1) translation and elevating CDK1 translation


Figure 1. NSun2 regulates p27 expression. (A) HeLa cells were transfected with a vector expressing NSun2 (pNSun2) or with a siRNA targeting NSun2. Forty-eight hours later, cell lysates were prepared and subjected to Western blot analysis to assess the levels of proteins NSun2, p27, and GAPDH. (B) RNA prepared from cells described in Fig. 1A was used for RT-qPCR analysis to assess the levels of p27 mRNA. Data represent the means ± SD from 3 independent experiments.