Figure 4. Changes in the TGFβ downstream signaling proteins in wounds of AT1R−/− and AT2R−/− mice. AT1R−/− mice have lower expression of Smad3 and the common mediator Smad4 in healed skin (day20) as compared to WT. A decrease in the expression of Smad3 was also observed in the AT2R−/− mice. The photomicrographs presented in red fluorescent staining with a blue DAPI counter stain for nuclei at 10x magnification. Quantification of mean fluorescence intensity of Smads in wild-type and mutant mice is shown. Scale bar 200 μm. **p<0.005, ***p<0.0005.